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To lose is human, to win is fortune, but to try is our destiny. EARL C. SUITOR, JR. The idea of a Symposium on ’Arthropod Cell Cultures’ started in July of 1969 shortly after the untimely death of our colleague, EARL C. SUITOR, JR. , at the age of 38. At first we thought an afternoon or evening session would be sufficient, but we were soon convinced that the scope of the Symposium should be greatly enlarged. Interest in this topic was increasing at an astonishing rate. Since EARL SUITOR had made a distinct imprint in this new field, many scientists who knew him well or just casually wished to honor him in this manner. EARL SUITOR was born and raised in New England and received a B. S. degree in bacteriology from the University of Massachusetts in 1952. As a spirited young man, he enlisted in the U. S. Navy to see the world. Instead, he was assigned for most of his four-year ’hitch’ to the Naval Medical Research Institute. I met him there in 1954, an enthusiastic and imaginative young fellow with many interests, an avid reader of the classics, an occasional writer of poetry, who blended his interest in scientific literature with that of Science Fiction. In 1956, EARL left the Navy to attend George Washington University, where he earned an M. S. degree in 1958 and a Ph. D. degree in 1963.
1 The Culture of Cells from Insects and Ticks.- I. Cultivation of Dipteran Cells in Vitro.- II. Culture of Cells of the Flesh Fly, Sarcophaga bullata.- III. Establishment and Characterization of Two New Cell Lines (CP-1268 and CP-169) from the Codling Moth, Carpocapsa pomonella (with a Review of Culture of Cells and Tissues from Lepidoptera).- IV. A Cell Line Isolated from Hemocytes of Samia cynthia Pupae.- V. Present Status of Tick Tissue Culture.- 2 Analysis of Cells from Established Insect Cell Lines.- I. Introduction.- II. Morphological and Cultural Characteristics of Culiseta inornata and Aedes vexans Mosquito Cell Lines.- III. Antigenic Relationships of Mosquito Cell Lines as Determined by Immunodiffusion Techniques.- IV. Characterization and Identification of Insect Cell Cultures.- V. Cytogenetic Studies on Cells in Culture from the Class Insecta.- 3 Physiology of Cultivated Arthropod Cells.- I. Introduction.- II. Drosophila Neuron Differentiation in Vitro.- III. Cultivation of Insect Endocrine Glands in Vitro.- IV. Production of Human-Mosquito Somatic Cell Hybrids and their Response to Virus Infection.- V. The Uptake of Free and Serum-Bound Sterols by Insect Cells in Vitro.- VI. Comparative Lipid Analysis of Aedes aegypti and Monkey Kidney Cells (MK-2) Cultivated in Vitro.- VII. Studies Attempting to Elucidate the in Vivo Function(s) of Grace’s Antheraea eucalypti Cell Strains.- VIII. The Effects of Insecticides on Insect Cells Grown in Vitro.- 4 Arthropod Tissue Culture in the Study of Arboviruses and Rickettsiae: A Review.- A. Arbovirus Studies.- B. Rickettsial Studies.- C. Concluding Remarks.- 5 Propagation of Arboviruses in Singh’s Aedes Cell Lines.- I. Growth of Arboviruses in Aedes albopictus and A. aegypti Cell Lines.- II. Multiplication of Chikungunya and O’nyong-nyong Viruses in Singh’s Aedes Cell Lines.- III. Growth Studies of California Encephalitis Virus in Two Aedes Mosquito Cell Line Cultures.- 6 Growth of Arboviruses in Arthropod Cell Cultures: Comparative Studies.- I. Preliminary Observations on Growth of Arboviruses in a Newly Established Line of Mosquito Cell (Culex quinquefasciatusSay).- II. Attempts to Grow Tacaribe and Junin Viruses in Grace’s Continuous Line of Moth Cells.- III. A Comparative Study of the Viral Susceptibility of Monolayer and Suspended Mosquito Cell Lines.- 7 Growth of Viruses in Arthropod Cell Cultures: Applications.- I. Attenuation of Semliki Forest (SF) Virus in Continuously Cultured Aedes aegypti Mosquito Cells (Peleg) as a Step in Production of Vaccines.- II. Cultivation of Oncogenic Viruses in Mosquito Cells in Vitro.- III. RNA of Uninfected and Sindbis Virus-Infected Aedes albopictus Cells.- 8 Homoptera Cell Culture and Its Application to the Study of Plant Pathogens.- II. In Vitro Cultivation.- III. Application to the Study of Plant Pathogens.- 9 Lepidoptera Cell Culture and Its Application to the Study of Plant Viruses and Animal Parasites.- I. Cultivation of Gypsy Moth Hemocytes.- II. Growth of a Microsporidian Parasite in Cultured Cells of Tent Caterpillars (Malacosoma).- III. Drosophila Cells in Vitro: Behavior and Utilization for Genetic Purpose.- 10 Drosophila Cell Culture and Its Application for the Study of Genetics and Virology.- I. Drosophila Cells in Vitro: Behavior and Utilization for Genetic Purpose.- II. Established Diploid Cell Lines of Drosophila melanogaster as Potential Material for the Study of Genetics of Somatic Cells.- III. Arbovirus Multiplication in an Established Diploid Cell Line of Drosophila melanogaster.- IV. Sigma Virus Multiplication in Drosophila Cell Lines of Different Genotypes.- V. The SR Condition in Drosophila bifasciata: In Vivo and in Vitro Growth.- 11 New Opportunities in Biological Research Offered by Arthropod Cell Cultures.- I. Some Speculations on the Possible Role of Arthropods in the Evolution of Arboviruses.- II. Future Possibil
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