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Preface Helder Maiato and Melina Schuh 1. Mitotic live-cell imaging at different timescales Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi 2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture Nicholas I. Clarke and Stephen J. Royle 3. Quantification of three-dimensional spindle architecture Norbert Lindow, Stefanie Redemann, Florian Brunig, Gunar Fabig, Thomas Muller-Reichert and Steffen Prohaska 4. Quantitative live and super-resolution microscopy of mitotic chromosomes Nike Walther and Jan Ellenberg 5. Live imaging of cell division in 3D stem-cell organoid cultures Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, Rene M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops 6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide Iskra Yanakieva, Marija Matejcic and Caren Norden 7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants Petra Vyplelova, Miroslav Ovecka, George Komis and Jozef Samaj 8. Microsurgery and microinjection techniques in mitosis research Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe 9. Analyzing the micromechanics of the cell division apparatus Yuta Shimamoto and Tarun M. Kapoor 10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells Ana Milas, Mihaela Jagric, Jelena Martincic and Iva M. Tolic 11. Live imaging of C. elegans oocytes and early embryos Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont 12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes Pierre Rome and Hiroyuki Ohkura 13. Methods to study meiosis in insect spermatocytes James R. LaFountain, Jr and Christopher S. Cohan 14. Live-cell microscopy of meiosis in spermatocytes Hiroki Shibuya and Yoshinori Watanabe 15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg 16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Peter Lenart 17. A microscopy-based approach for studying meiosis in live and fixed human oocytes Agata P. Zielinska and Melina Schuh 18. Superresolution imaging of the synaptonemal complex Katharina Schucker, Markus Sauer and Ricardo Benavente 19. Cryoelectron tomography of SYCP3 fibres under native conditions Daniel Bollschweiler, Laura Radu and Luca Pellegrini

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