A good text for the amateur bioinformatician or the journeyman looking to improve their overall approach. Not meant for the novice with little command line experience. The book provides an overview of many broad topics as opposed to specific tools, and the author does a good job of emphasizing reproducibility, which is surprisingly poorly practiced in the field. Recommended.

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Well written text. An excellent chapter with mathematical examples on the optical design of the eye. A must for any reader interested in the topic.

About the Author M. J. Kidnie is a professor at the University of Western Ontario, Canada. She has edited several early modern works of prose and drama, and her research currently centres on live performance and adaptation.

SEAN CARROLL is a theoretical physicist at the California Institute of Technology, host of the Mindscape podcast, and author of From Eternity to Here, The Particle at the End of the Universe, and The Big Picture. He has been awarded prizes and fellowships by the National Science Foundation, NASA, the American Institute of Physics, and the Royal Society of London, among many others. He lives in Los Angeles with his wife, writer Jennifer Ouellette.

This book has most useful practical problem sets about atomic physics. All the problems are related to real experiments. One can learn both theories and experimental methods from them. Most importantly, this book provides lots of useful \"numbers\" that a physicists should memorize. For physics graduate students, reading this book is a good way to review what you have learned in quantum mechanics class and to prepare for prelim qualifying exams.

The title is a stopper, and meant to be. The book is extremely sophisticated, detailed and convincing. 100 or even 50 years ago, no one could have made a case for plant intelligence, but dramatic research findings over the last decades have shown that plants--although they lack nervous systems--have exceedingly complex internal communication, mediated by chemicals (plant hormones, ions, etc.). Trewavas provides full details on the chemistry. There is communication even within cells, but what is impressive is the amount of communication and even decision-making that goes on in the whole plant. It has to decide how to grow toward light and away from shade, toward water and nutrients and away from sterile soil, etc. We once thought this was a simple, automatic process, but the reality has turned out to be as chemically and strategically complicated as decision-making in simple invertebrate animals.

本书是城市气候领域第一部综合现代科学研究与实用研究的著作。书的开端介绍了城市生态系统的构成要素。并以城市的地表与范围分类为基础，制订了城市气候方面的综合性术语。本书解释了形成若干典型城市气候的主要物理原因，如环建筑物气流、热岛、降雨干预与空气污染。并且展示了如何利用各种因素去改变城市发展带来的不良后果，构建一个可持续发展的、复原力强的城市，近来城市气候研究越来越成熟，而这部应时出版的作品将城市气候研究的不同领域纳入一个框架之中，是气候学、城市水文地理学、空气质量、环境工程和城市设计领域内学生和研究者的理想读物。