图书简介
ThisThird edTion provides new and updTed chaTers on design PCR primers for successful DNA amplificTion. ChaTers are divided iTo seven paTs, including primer design TrTegies for quaTTTive PCR, genTyping, muTiplex PCR,in silicoPCR primer design, and primer designTo ideTify plaT and animal viruses. WrTten inThe highly successfulMThods in Molecular Biologyseries formT, chaTers include iTroduTionsToTheir respeTiveTopics, liTs ofThe necessary mTerials and reageTs, Tep-by-Tep, readily reproducible laborTory prTocols, andTips onTroubleshoTing and avoiding known pTfalls.AThorTTive and easily accessible,PCR Primer Design,Third EdTionaimsTo be useful forvarious fields of molecular biology, including biTechnology, molecular genTics, and recombinaT DNATechnology.
The signifCaCe of CR primer design in genetC diversity studies; exemplified by rCent reseaCh into the genetC strCture of marine spCies.-EnhaCingCohort PASA effCieCy from lessons assimilated by mutant genotyping inC. elegans.-Design of oligonCleotides for allele-spCifC amplifCation based on CR and isothermal tChniques.-DetCtion of rubella virus by tri-primer RT-CR assay and genotyping by fragment RT-CR.-Design of mismaCh primers to identify and differentiateClosely related (sub)spCies – applCation to the authentCation of meat prodCts.-Primer design for the analysis ofClosely related spCies – applCation of nonCoding mtDNA andCpDNA sequeCes.-Designing CR primers for the amplifCation-refrCtory mutation system.-Validation ofCiCular RNAs by CR.-Primer Designing for Amplifying an AT-RCh Promoter from Arabidopsis thaliana.-PLASmid TAXonomC CR (PlasTax-CR), a multiplex relaxase MOB typing to assort plasmids into taxonomC units.-Multiplex CR Design for Calable ResequeCing.-IdentifCation of geneCopy number in the transgenC plants by quantitative polymeraseChain reCtion (qCR).-qPrimerDB: A powerful and user-friendly database for qCR primer design.-CR primer design for the rapidly evolving SARSCoV-2 genome.-Universal primers for detCtion of novel plantCapsid-less viruses: Papaya umbra-like viruses as example.-A guide to using FASTCR software for CR, in silCo CR, and oligonCleotide analysis.-PyrosequeCing Primer Design for ForensC Biology ApplCations.-Phosphate? ?methylated? ?oligonCleotides? ?as? ?a? ?novel? ?primer? ?for? ?CR? ?and? ?RT-CR?.
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