图书简介
This second edition volume expands on the previous edition with updates about the latest state-of-the-art techniques used in leading hemostasis and thrombosis laboratories for diagnosis and exclusion of hemorrhagic and thrombotic diseases. The chapters in this book are organized into seven parts. Part One provides a general overview on hemostasis and thrombosis, preanalytical issues in testing, and routine hemostasis assays. Part Two covers laboratory testing for thrombophilia, including reviews for activated protein C resistance, protein C, lupus anticoagulant testing, and antiphospholipid antibodies. Part Three addresses monitoring continuous anticoagulant infusions and measuring the effects of oral anti-thrombotic therapy. Part Four talks about heparin induced thrombocytopenia and vaccine induced immune thrombotic thrombocytopenia. Part Five and Six cover ADAMTS13 activity testing and new information on bleeding disorders such as chromogenic factor VIII assays, measurement of emicizumab, and treatment of hemophilia A and B. Finally, Part Seven discusses global assays, research applications, and postanalytical considerations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Hemostasis and Thrombosis: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers struggling to identify the appropriate methods for hemostasis and thrombosis testing, or who seek additional expert guidance on such testing.
Hemostasis and Thrombosis: An Overview Focusing on Associated Laboratory Testing to Diagnose and Help Manage Related Disorders.- Preanalytical Variables in Hemostasis Testing.- Automation in the Thrombosis and Hemostasis Laboratory.- Ultracentrifugation for Coagulation Testing.- Harmonization of Hemostasis Testing Across a Large Laboratory Network: An Example from Australia.- Auto-Validation of Routine Coagulation/Hemostasis Assays with Reflex Testing of Abnormal Test Results.- Laboratory Testing of Hemostasis in Pregnancy: A Brief Overview.- Protocols for D-Dimer Measurements for Aid to Diagnosis or Exclusion of Venous Thromboembolism.- Performance and Interpretation of Clot Waveform Analysis.- Laboratory Evaluation of Thrombophilia.- Laboratory Testing for Activated Protein C Resistance (APCR): An Update.- Evaluation of Activated Protein C Resistance using Thrombin Generation Test.- Performance of Chromogenic Protein C (PC) Testing.- Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants.- An Overview of Laboratory Testing for Antiphospholipid Antibodies.- Lupus Anticoagulant Testing: Taipan Snake Venom Time with Ecarin Time as Confirmatory Test.- Lupus Anticoagulant Testing: Dilute Prothrombin Time (dPT).- An Acute Need Inspiration: Autoneutralization of Lupus Anticoagulants in Affected Prothrombin Times (PT) and Activated Partial Thromboplastin Times (APTT).- Antiphospholipid Antibody Testing for Anti-Cardiolipin and Anti-2Glycoprotein I Antibodies using Chemiluminescence-Based Panels.- Laboratory Testing for Non-Criteria Antiphospholipid Antibodies-Antiphosphatidylserine/Prothrombin Antibodies (aPS/PT).- Laboratory Testing for Non-Criteria Antiphospholipid Antibodies: Antibodies Towards the Domain One of Beta2-Glycoprotein I (aDI).- An Overview of Heparin Monitoring with the Anti-Xa Assay.- Ecarin Based Methods for Measuring Thrombin Inhibitors.- Assays to Monitor Bivalirudin.- Monitoring of Anti-Platelet Therapy.- Heparin Induced Thrombotic Thrombocytopenia (HITT) and Vaccine Induced Immune Thrombotic Thrombocytopenia (VITT): Similar by Different.- Functional Testing for Heparin Induced Thrombocytopenia using Whole Blood Multiplate Impedance Aggregometry.- Multiple Electrode Aggregometry (Multiplate): Functional Assay for Vaccine Induced (Immune) Thrombotic Thrombocytopenia (VITT).- Whole Blood Procoagulant Platelet Flow Cytometry Protocol for Heparin-Induced Thrombocytopenia (HIT) and Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) Testing.- Serotonin Release Assay: Functional Assay for Heparin and Vaccine Induced (Immune) Thrombotic Thrombocytopenia.- An Overview of Laboratory Testing for ADAMTS13.- Automated and Rapid ADAMTS13 Testing using Chemiluminescence: Utility for Identification or Exclusion of TTP and Beyond.- Identification of ADAMTS13 Inhibitors in Acquired TTP.- ADAMTS13 Activity: Screening Test Protocol.- ADAMTS13 Activity Measurement by ELISA and Fluorescence Resonance Energy Transfer Assay.- ADAMTS13 Antibody and Inhibitor Assays.- A Review of Factor VIII and Factor IX Assay Methods for Monitoring Extended Half-Life Products in Haemophilia A and B.- Measuring Emicizumab Levels in the Hemostasis Laboratory.- Chromogenic Factor VIII Assay for Patients with Haemophilia A and On Emicizumab Therapy.- Assessment of Platelet Function by Automated Light Transmission Aggregometry.- Assessment of Platelet Function by High-Throughput Screening Light Transmittance Aggregometry: Optimul Assay.- Preparing Surrogate Abnormal Quality Samples for Platelet Function Studies and Viscoelastic Testing.- Laboratory Testing for von Willebrand Disease Using a Composite Rapid 3-Test Chemiluminescence-Based von Willebrand Factor Assay Panel.- Laboratory Testing for von Wilebrand Factor Activity by a Glycoproein Ib Binding Assay (VWF:GPIbR): HemosIL von Willebrand Factor Ristocetin Cofactor Activity on ACL TOP®.- Laboratory Testing for von Willebrand Factor:Factor VIII Binding for Diagnosis or Exclusion of Type 2N von Willebrand Disease: An Update.- Automation of a Factor XIII Activity Assay Utilizing a Plasma Blank Measurement.- Measurement of Blood Viscoelasticity using Thromboelastography.- The TEG 5000© System: System Description and Protocol for Measurements.- The TEG 6s© System: System Description and Protocol for Measurements.- The Quantra® System: System Description and Protocols for Measurements.- Modified Rotational Thromboelastometry Protocol using Tissue Plasminogen Activator for Detection of Hypofibrinolysis and Hyperfibrinolysis.- Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness using Scanning Electron Microscopy.- Post-Analytical Issues in Hemostasis and Thrombosis Testing: An Update.
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