Ras Activity and Signaling

图书简介

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

Ras Variant Biology and Contributions to Human Disease.- Regulation of the Small GTPase Ras and its Relevance to Human Disease.- Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry.- Absolute Quantitation of GTPase Protein Abundance.- Validation of Isoform- and Mutation-specific RAS Antibodies.- Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b.- Active GTPase Pulldown Protocol.- Methods to Monitor Ras Activation State.- NMR Detection Methods for Profiling RAS Nucleotide Cycling.- Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations.- Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations.- Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets.- FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage.- Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry.- Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA).- Using BioID to Characterize the RAS Interactome.- Probing RAS function with Monobodies.- Detection of Endogenous RASSF1A Interacting Proteins.- Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition.- A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background.- RASless MEFs as a Tool to Study RAS-dependent and -independent Functions.- Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies.- Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling .- Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation.- Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models.- Ras, Ral, and Rap1 in C. elegans.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Ras Activity and Signaling（Methods in Molecular Biology） 肾素活性与信号传导：方法与协议 生物化学

Ras Activity and Signaling（Methods in Molecular Biology）

肾素活性与信号传导：方法与协议
生物化学