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Preface About the Editors Contributors PART I: PRACTICAL INFORMATION AND PROCEDURES 1. Sterilization, Disinfection, and Antisepsis Michael G. Schmidt 2. Quantitation of Microorganisms Brad A. Slominski and Peter S. Lee 3. Culturing and Preserving Microorganisms Lorrence H. Green 4. Stains for Light Microscopy Stuart Chaskes and Rita Austin 5. Identification of Gram-Positive Organisms Peter M. Colaninno 6. Identification of Aerobic Gram-Negative Bacteria Donna J. Kohlerschmidt, Lisa A. Mingle, Nellie B. Dumas, and Geetha Nattanmai 7. Plaque Assay for Bacteriophage Emanuel Goldman 8. Phage Identification of Bacteria Catherine E.D. Rees and Martin J. Loessner 9. Phage Display and Selection of Protein Ligands Geir Age Loset, Wlodek Mandecki, and Inger Sandlie 10. Diagnostic Medical Microbiology Lorrence H. Green 11. Modern Diagnostic Methods in the 21st Century Lorrence H. Green and Alan Ward 12. Antibiotic Susceptibility Testing Audrey Wanger and Violeta Chavez 13. Bacterial Cell Breakage or Lysis Matthew E. Bahamonde 14. Major Culture Collections and Sources Lorrence H. Green 15. Epidemiological Methods in Microbiology Tyler S. Brown, Barun Mathema, and D. Ashley Robinson 16. CRISPR Tao Xu, Megan L. Kempher, Xuanyu Tao, Aifen Zhou, and Jizhong Zhou PART II: SURVEY OF MICROORGANISMS 17. Taxonomic Classification of Bacteria J. Michael Janda 18. Bacterial Cell Wall: Morphology and Biochemistry Stefania De Benedetti, Jed F. Fisher, and Shahriar Mobashery 19. The Human Microbiome in Health and Disease Sandra B. Andersen, Menghan Liu, and Martin J. Blaser 20. The Phylum Actinobacteria Alan C Ward, Nagamani Bora, Jenileima Devi, Alexander Escasinas, and Nicholas Allenby 21. Archaea Nina Dombrowski, Tara Mahendrarajah, Sarah T. Gross, Laura Eme, and Anja Spang 22. The Genus Bacillus Daniel R. Zeigler and John B. Perkins 23. The Genus Bordetella Rita Austin and Tonya Shearin-Patterson 24. The Genus Campylobacter Collette Fitzgerald, Janet Pruckler, Maria Karlsson, and Patrick Kwan Updated 2019: Janet Pruckler, Lavin Joseph, Hayat Caidi, Mark Laughlin, Rachael D. Aubert 25. Chlamydiae Lourdes G. Bahamonde 26. The Genus Clostridium Peter Durre 27. The Genus Corynebacterium Lothar Eggeling and Michael Bott 28. The Family Enterobacteriaceae J. Michael Janda and Denise L. Lopez 29. Haemophilus Species Elisabeth Adderson 30. The Genus Helicobacter Ernestine M. Vellozzi and Edmund R. Giugliano 31. The Genus Legionella Ashley M. Joseph and Stephanie R. Shames 32. The Genus Listeria Sukhadeo Barbuddhe, Torsten Hain, Swapnil P. Doijad, and Trinad Chakraborty 33. The Genus Mycobacterium Leen Rigouts and Sari Cogneau 34. Mycoplasma and Related Organisms Bahman Rostama and Meghan May 35. The Family Neisseriaceae Yvonne A. Lue 36. The Genus Pseudomonas Layla Ramos-Hegazy, Shubham Chakravarty, and Gregory G. Anderson 37. The Family Rickettsiaceae Timothy P. Driscoll, Victoria I. Verhoeve, Magda Beier-Sexton, Abdu F. Azad, and Joseph J. Gillespie 38. Microbiological and Clinical Aspects of the Pathogenic Spirochetes Charles S. Pavia 39. Staphylococcus aureus and Related Staphylococci Volker Winstel, Olaf Schneewind, and Dominique Missiakas 40. Streptococcus Vincent A. Fischetti and Patricia Ryan 41. The Genus Vibrio and Related Genera Seon Young Choi, Anwar Huq, and Rita R. Colwell 42. Yersinia Ryan F. Relich and Meghan A. May 43. Other Anaerobic Bacteria: Bacteroides, Porphyromonas, Prevotella, Tannerella, Fusobacterium, and Gram-positive Anaerobic Cocci Joseph J. Zambon and Violet I. Haraszthy 44. Other Gram-Negative Bacteria: Acinetobacter, Burkholderia, and Moraxella Rebecca E. Colman and Jason W. Sahl 45. Selected Zoonotic Pathogens Sanjay K. Shukla and Steven Foley 46. Fungi Charles Adair 47. Introduction to Parasites Purnima Bhanot 48. Introduction to Bacteriophages Elizabeth Kutter and Emanuel Goldman 49. Introduction to Virology Ken S. Rosenthal 50. Emerging Viruses Meghan A. May and Ryan F. RelichContents PART III: APPLIED PRACTICAL MICROBIOLOGY 51. Mechanisms of Action of Antibacterial Agents Ammara Mushtaq, Joseph Adrian L. Buensalido, Carmen E. DeMarco, Rimsha Sohail, and Stephen A. Lerner 52. Mechanisms of Action of Antifungal Agents Jeffrey M. Rybak and P. David Rogers 53. Mechanisms of Action of Antiviral Agents Guido Antonelli, Francesca Falasca, and Ombretta Turriziani 54. Phage Therapy: Bacteriophages as Natural, Self-Replicating Antimicrobials Naomi Hoyle and Elizabeth Martin Kutter 55. Emergence of Antimicrobial Resistance in Hospitals Paramita Basu, Joshua Garcia, and Priyank Kumar 56. Emerging Antimicrobial-Resistant Microorganisms in the Community Negin Alidazeh Shaygh, Divya Sarvaiya, and Paramita Basu 57. Overview of Biofilms and Some Key Methods for Their Study Paramita Basu, Michael Boadu, and Irvin N. Hirshfield 58. Biofilms in Healthcare Rebecca K. Kavanagh, Arindam Mitra, and Paramita Basu 59. The Business of Microbiology Michael C. Nugent and Lorrence H. Green 60. Launching a Microbiology-Based Company Leonard Osser 61. Microbiology for Dental Hygienists Victoria Benvenuto and Donna L Catapano 62. Microbiology for Pre-College Teachers Madge Nanney and Scott Sowell 63. Microbiology for Home Inspectors William E. Herrmann Survey of Selected Clinical, Commercial, and Research-Model Eubacterial Species Index

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