Protein Self-Assembly:Methods and Protocols

图书简介

This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

Measuring Non-Specific Protein-Protein Interactions by Dynamic Light Scattering.-Light Scattering to Quantify Protein-Protein Interactions at High Protein Concentrations.-Quantitative Evaluation of Protein Solubility in Aqueous Solutions by PEG-Induced Liquid-Liquid Phase Separation.-Measuring Protein Solubility.-Integral caa3-Cytochrome c Oxidase from Thermus thermophiles: Purification and Crystallization.-Aggregation Profiling of C9orf72 Dipeptide Repeat Proteins Transgenically Expressed inDrosophila melanogasterusing an Analytical Ultracentrifuge Equipped with Fluorescence Detection.-Sample Preparation and Size Analysis of C9orf72 Dipeptide Repeat Proteins Expressed in Drosophila melanogaster using Semi-Denaturing Agarose Gel Electrophoresis.-The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins.-Differential Scanning Calorimetry to Quantify Heat Induced Aggregation in Concentrated Protein Solution.- Nanoparticle Tracking Analysis to Examine the Temperature-Induced Aggregation of Proteins.- Evaluation of Temporal Aggregation Processes using Spatial Intensity Distribution Analysis.- Fluorescence Correlation Spectroscopy for Particle Sizing in Highly Concentrated Protein Solutions.-Size Determination of Protein Oligomers/Aggregates Using Diffusion NMR Spectroscopy.-Patchy Particle models to Understand Protein Phase Behavior.-Obtaining Soft Matter Models of Proteins and their Phase Behavior.-Binding Free Energies of Conformationally Disordered Peptides through Extensive Sampling and End-Point Methods.-Atomistic Simulation Tools to Study Protein Self-Aggregation.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Protein Self-Assembly:Methods and Protocols（Methods in Molecular Biology） 生物化学

Protein Self-Assembly:Methods and Protocols（Methods in Molecular Biology）
生物化学