图书简介

The Essential, Ubiquitous Single-Stranded DNA-binding Proteins.-Single-stranded DNA Binding Proteins in the Archaea.-Single-molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes.-Comparing SSB-PriA Functional and Physical Interactions in Gram-positive and -Negative Bacteria.-In vivo binding of Single-stranded DNA-binding Protein to Stalled Replication Fork Helicases.-Magnetic Tweezers-based Single Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase.-High-throughput Screening to Identify Inhibitors of SSB-Protein Interactions.-Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly.-Generation of Fluorescent Versions of Saccharomyces cerevisiae RPA to Study the Conformational Dynamics of its ssDNA-binding Domains.-RPA-1 from Leishmania sp.: Recombinant Protein Expression and Purification, Molecular Modeling and Molecular Dynamics Simulations Protocols.-Single-stranded DNA Curtains for Single-molecule Visualization of Rad51-ssDNA Filament Dynamics.-Following Trypanosoma cruzi RPA-DNA Interaction Using Fluorescent in Situ Hybridization Coupled with Immunofluorescence (FISH/IF).-Quantifying the affinity of Trypanosoma cruzi RPA-1 to the Single-stranded DNA Overhang of the Telomere using Surface Plasmon Resonance.-Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-stranded DNA Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A).-Atomic Force Microscopy Reveals that the Drosophila Telomere-Capping Protein Verrocchio is a Single-stranded DNA Binding Protein.-Analysis of Mitochondrial SSB-DNA Complexes and their Effects on DNA Polymerase ? Activity by Electron Microscopy and Enzymatic Assays.-Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-binding Protein-DNA Complexes.-Measurements of Real-time Replication Kinetics of DNA Polymerases on ssDNA Templates Coated with Single-stranded DNA-binding Proteins.-Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophila Schneider S2 cells.-Stimulation of Variant forms of the Mitochondrial DNA Helicase Twinkle by the Mitochondrial Single-stranded DNA-binding Protein.-Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome.-Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB during Phi29 TP-DNA rReplication.-Structural Characterization of a Single-stranded DNA-binding Protein: A Case Study of the ORF6 protein from Bacteriophage Enc34.

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